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IMAGING of
NUCLEIC ACIDS
and QUANTITATION
in PHOTONIC
MICROSCOPY
Methods in Visualization
Series Editor: Gérard Morel
In Situ Hybridization in Light Microscopy
Gérard Morel and Annie Cavalier
Visualization of Receptors: In Situ Applications
of Radioligand Binding
Emmanuel Moyse and Slavica M. Krantic
Genome Visualization by Classic Methods
in Light Microscopy
Jean-Marie Exbrayat
Imaging of Nucleic Acids and Quantitation
in Photonic Microscopy
Xavier Ronot and Yves Usson
In Situ Hybridization in Electron Microscopy
Gérard Morel, Annie Cavalier,
and Lynda Williams
NUCLEIC ACIDS
and QUANTITATION
in PHOTONIC
MICROSCOPY
Edited by
Xavier Ronot
Professor at the Ecole Pratique des Hautes Etudes
Yves Usson
Senior Researcher at the
Centre National de la Recherche Scientifique
CRC Press
Boca Raton London New York Washington, D.C.
IMAGING of
Cover inset: Confocal 3-D reconstruction of HeLa cell mitotic nuclei after heat shock treatment. chromosomes
are shown in red, and HSF1 transcription factors are shown in green. For details, see Jolly, Usson, and
Morimoto, PNAS, 1998, 96: 6769–6774.
Library of Congress Cataloging-in-Publication Data
Imaging of nucleic acids and quantitation in photonic microscopy / edited by Yves Usson,
Xavier Ronot.
p. cm. — (Methods in visualization)
ISBN 0-8493-0817-8 (alk. paper)
1. Nucleic acids—Analysis. 2. Fluorescent probes. 3. Fluorescence microscopy. I.
Usson, Yves. II. Ronot, Xavier. III. Series.
QP620 .I43 2001
572.8'028—dc21
00-140132
CIP
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